Applications for GS FLX technology

De Novo Genome Sequencing

• Whole genomes of Plants, Yeasts, Fungi, Bacteria, Viruses
• BACs, Fosmids, Plastids

Resequencing

• Whole genomes of Human, Plants, Yeasts, Fungi, Bacteria, Viruses
• BACs, Fosmids
• Genomic Rearragements
• Disease Associated Regions
• Single Nucleotide Polymorphisms
• Somatic Mutations

Paired-End Sequencing

• Whole genomes of Plants, Yeasts, Fungi, Bacteria, Viruses

Transcriptome Analysis

• Full-length transcripts
• Expressed Sequence Tags (ESTs)
• 5'-Transcript Ends (5'-RATE)
• Multiplex Sequencing of Paired-End Ditags (Singapore MS-PET)
• Serial Analysis of Gene Expression (SAGE)
• Genome identification sequencing (GIS-PET)

Gene Regulation Studies

• Small non-coding RNAs
• Transcription Factor Binding Sites (ChIP-Sequencing)
• Analysis of DNA methylation patterns

Epigenetic Changes

• DNA methylation pattern
• Nucleosome Modifications

Metagenomics and Microbial Diversity

• Analysis of Environmental DNA
• 16s rRNA

Paleogenomics

• Whole genome sequencing of ancient DNA

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