Arrangements must be made in advance for sequencing. These arrangements will include scheduling details and a date for sample submission. A project request form should be submitted using our submission form. Once all arrangements have been finalized and the sample has been received by our sequencing facility, we will provide the user with the sequence data and a draft assembly within 4-6 weeks. Failure to submit samples on time will result in scheduling delays and sequencer inactivity. If this occurs the cost of a single maintenance wash kit will be charge to the user for each week of delay.

Genomic Sample Submission

• The use of Qiagen's Genomic DNA Prep Kit for DNA isolation.
• ≥5 ug of gDNA in 100 ul of TE Buffer in a 1.5ml tube with the sample name and concentration (ug/ul) visibly written on the tube. If available, please provide an estimation of the genome size and/or the name and size of a reference genome.
• Quality control documentation should also be submitted with the sample.
• An image of the sample run on an agarose gel including qualitative and quantitative ladders and results from a PicoGreen Assay verifying sample concentration.
• A spectrophotometer reading also verifying concentration with the appropriate 260/230 and 260/280 readings: 1.5 and 1.7 respectively.
• Once the above requirements are met, we will begin library preparation for a sequencing run. Throughout the library preparation process, we will conduct our own quality control measures to determine if the single-stranded library produced will be sufficient for the run specified. We reserve the right to discontinue the preparation of the sample if our own quality control measures are not met. If this were to occur, you will be responsible only for the cost of library preparation. However, if we do a sequencing run and the sample fails, we will run it again without any extra cost to you.

Amplicon Sample Submission

• 10-50 ng for a complex amplicon sample (ie/ genomic DNA) or 1-5 ng for cloned/PCR-generated templates containing specified fusion primers in a 1.5ml tube with the sample name and concentration (ng/ul) visibly written on the tube.
• Quality control documentation should also be submitted with the sample.
• An image of the sample run on an agarose gel including qualitative and quantitative ladders and results from a PicoGreen Assay verifying sample concentration.
• A spectrophotometer reading also verifying concentration with the appropriate 260/230 and 260/280 readings: 1.5 and 1.7 respectively.
• Once the above requirements are met, we will begin library preparation for a sequencing run. Throughout the library preparation process, we will conduct our own quality control measures to determine if the single-stranded library produced will be sufficient for the run specified. We reserve the right to discontinue the preparation of the sample if our own quality control measures are not met. If this were to occur, you will be responsible only for the cost of library preparation. However, if we do a sequencing run and the sample fails, we will run it again without any extra cost to you.

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